Molecular and Technological Characterization of Saccharomyces cerevisiae from Sourdough

DNA markers help detect the intraspecific genetic diversity of yeast strains. Eight ISSR (Inter Simple Sequence Repeats) primers were used to assess Saccharomyces cerevisiae (n = 96) from different populations 3), evaluate technological characteristics, and investigate trait-loci associations. The amplified 154 reproducible scorable bands, which 79.87% polymorphic. UPGMA (unweighted pair group method with arithmetic mean) dendrogram clustered 96 isolates into two main clusters, supported by STRUCTURE HARVESTER results (ΔK 2). Analysis molecular variance (AMOVA) indicated significant differences between (15%) within (85%) (p < 0.001). Twenty-nine genetically distinct strains selected for characterization. Principal component analysis (PCA) revealed that five high fermentation capacity, leavening activity, growth index at 37 °C, harsh conditions technologically relevant. Trait-loci association analyses highest correlation (r 0.60) was recorded capacity on 8th 113th loci, ISSR-1 ISSR-6 primers, respectively 0.05). yielding performances associated loci possess a potential generate locus-specific target capacity.